Hep 3B-Luc cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

Hep 3B-Luc Order

Catalog#

YC-D002-Luc-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium(Hep 3B)

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Luciferase Stable Cell Lines

cell name

Hep 3B-Luc

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Reporter gene

Luciferase

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

87%MEM+10% FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA

Luciferase validation

Sample	Compound pore 1	Compound pore 2	Compound pore 3	Average	Expressed multiple
Hep 3B-Luc	134792	155597	144562	144983.667	162.903
Hep 3B	864	861	945	890.0	162.903

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hep 3B			STR Info (Cell bank) Cell Line:HEP-3B
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	12	14		12	14
D7S820	8	10		8	10
D16S539	10	10		10	10
VWA	17	17		17	17
TH01	6	7		6	7
AMEL	X	X		X	X
TPOX	9	9		9	9
CSF1PO	8	8		8	8

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use luciferase gene expressing lentivirus constructed by Ubigene to infect the Hep 3B cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Hep 3B-Luc cell line that can maintain long-term, stable and high expression.

Product Advantage

Easy to use: Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
01
Sensitive accurate：Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
02
High-quality cell: Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.
03

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